Desert Ants: The Magnetic Field Calibrates the Navigation System

They are only a few centimeters tall and their brains have a comparatively simple structure with less than one million neurons. Nevertheless, desert ants of the Cataglyphis genus  possess abilities that distinguish them from many other creatures: The animals are able to orient themselves to the Earth's magnetic field.

Visible Changes in the Nervous System

A research team from Julius-Maximilians-Universität Würzburg (JMU) discovered this a few years ago. However, it was previously unknown where in the ants' brains the magnetic information is processed. This has now changed: In a new study published in the journal PNAS - Proceedings of the National Academy of Sciences, the team shows that information about the Earth's magnetic field is primarily processed in the ants' internal compass, the so-called central complex, and in the mushroom bodies, the animals' learning and memory centers.

Professor Wolfgang Rössler, holder of the Chair of Behavioral Physiology and Sociobiology at the University of Würzburg, Dr. Pauline Fleischmann, former scientist at the Chair of Behavioral Physiology and Sociobiology and now a member of the Neurosensorics/ Animal Navigation working group at the University of Oldenburg, and Dr. Robin Grob, who has since moved from Rössler's chair to the Norwegian University of Science and Technology in Trondheim, were responsible for this study.

First Exploratory Walks for Calibration

"Before an ant leaves its underground nest for the first time and goes in search of food, it has to calibrate its navigation system," says Pauline Fleischmann, explaining the background to the work. During so-called learning walks, the animals then explore the immediate surroundings around the nest entrance and repeatedly pirouette around their own body axis with short stops in between. During these pauses, they always look exactly back in the direction of the nest entrance, even though they cannot see it – a tiny hole in the ground.

Thanks to their field studies in southern Greece, where Cataglyphis ants are native, Fleischmann and her colleagues were able to prove that desert ants orient themselves to the Earth's magnetic field during the learning walk phase. Pauline Fleischmann and Robin Grob were once again on site in Greece. This time, however, they not only investigated the ants' orientation behavior while the magnetic field was being manipulated, but also looked for changes in the nervous system of Cataglyphis as an expression of the newly acquired experience.

A Faulty Magnetic Field Disrupts the Learning Process

The zoologists concentrated on young workers that had not yet undertaken any learning walks. The animals were only allowed to set off as part of the precisely planned experiments – sometimes under natural conditions, sometimes in a permanently manipulated magnetic field that, for example, displayed chaotic directions or did not allow horizontal orientation. With this faulty directional information, it was not suitable as a reliable reference system for the ants' behavior to look back to the nest entrance during the learning walks.

The result: "Our neuroanatomical brain analyses show that ants exposed to an altered magnetic field have a smaller volume and fewer synaptic complexes in an area of the brain responsible for the integration of visual information and learning, the so-called mushroom body," explain Fleischmann and Grob. In the central complex, the region of the ant’s brain in which spatial orientation is anchored, the same findings were observed under certain conditions.

The Number of Synaptic Connections Increases

Desert ants that were allowed to make their first excursions under natural conditions were clearly different. Their sensory experiences, a combination of information about the magnetic field, the position of the sun and the visual environment, triggered a learning process that was accompanied by structural changes in the neurons and an increase in synaptic connections in the aforementioned brain regions.

According to the scientists, this leads to the conclusion that magnetic information not only serves as a compass for navigation, but also as a global reference system that is crucial for the formation of spatial memory.

In Search of the Sensory Organ

The results of their experiments prove "that ants need a functioning magnetic compass during their learning walks in order to calibrate their visual compass and at the same time store images of the nest environment in their long-term memory", as Pauline Fleischmann and Robin Grob say. At the same time, their research extends far beyond the field of compass calibration in ants. Wolfgang Rössler emphasizes that "the results provide valuable information on how multisensory stimuli can influence neuronal plasticity of brain circuits for navigation in a critical phase of brain maturation."

In a next step, the team now wants to investigate in which sensory organ the desert ant receives the magnetic information and via which sensory pathways it is transmitted and processed. This has not yet been achieved with any animal species that orients itself to the Earth's magnetic field. Due to their manageable and relatively small nervous system, insects, to which Cataglyphis belongs, offer a unique opportunity to investigate the neuronal basis of magnetic orientation at all levels.

Original Publication

Importance of Magnetic Information for Neuronal Plasticity in Desert Ants. Robin Grob, Valentin L. Müller, Kornelia Grübel, Wolfgang Rössler, Pauline N. Fleischmann. PNAS Online-Publikation vom 12.02.2024, https://doi.org/10.1073/pnas.2320764121

Contact

Prof. Dr. Wolfgang Rössler, University of Würzburg, T: +49 931 31-84306, roessler@biozentrum.uni-wuerzburg.de

Dr. Pauline N. Fleischmann, University of Oldenburg, T: +49 441 798-3743, pauline.fleischmann@uni-oldenburg.de

Dr. Robin Grob, Norwegian University of Science and Technology Trondheim, T: +47 406 27932, robin.grob@ntnu.no